Journal: International Journal of Molecular Sciences

Article Title: Development of 18 F-Labeled Radiotracers for PET Imaging of the Adenosine A 2A Receptor: Synthesis, Radiolabeling and Preliminary Biological Evaluation

doi: 10.3390/ijms22052285

Figure Lengend Snippet: ( A ) Representative radio- and UV-chromatograms obtained for isolation of [ 18 F] PPY1 [ReproSil-GOLD 120 C18 column (250 × 10 mm), 34% MeCN/H 2 O, flow rate: 4 mL/min] and [ 18 F] PPY2 [ReproSil-Pur 120 C18-AQ column (250 × 10 mm), 30% MeCN/H 2 O, flow rate: 5 mL/min] by semi-preparative RP-HPLC ( PPY22 = by-product); ( B ) radio- and UV-chromatograms of formulated [ 18 F] PPY1 and [ 18 F] PPY2 co-injected with the corresponding reference compound PPY1 or PPY2 [ReproSil-GOLD 120 C18 column (250 × 4.6 mm), 10–90–10% MeCN/H 2 O, flow rate: 1 mL/min].

Article Snippet: The purity of all the tested compounds was ≥95% as determined by an LC-MS system including a DAD detector [Dionex Ultimate 3000 system incorporating a LPG-3400SD pump, a WPS-3000 TSL autosampler, a TCC-3000SD column compartment, a DAD 3000 diode array detector and a MSQ Plus low resolution mass spectrometer (Thermo Fisher Scientific Inc., Waltham, MA, USA), column: Reprosil-Pur Basic HD (150 × 3 mm; 3 µm; Dr. Maisch GmbH, Ammerbuch, Germany), gradient: 10–90–10% MeCN/ 20 mM NH 4 OAc aq. ( v/v ), run time: 15 min, flow rate: 0.6 mL/min, UV-detection: 254 nm].

Techniques: Isolation, Injection

Journal: International Journal of Molecular Sciences

Article Title: Development of 18 F-Labeled Radiotracers for PET Imaging of the Adenosine A 2A Receptor: Synthesis, Radiolabeling and Preliminary Biological Evaluation

doi: 10.3390/ijms22052285

Figure Lengend Snippet: In vivo metabolism study in CD-1 mice: Representative radio-chromatograms of extracted ( A ) brain and ( B ) plasma samples at 30 min p.i. of [ 18 F] PPY1 [ReproSil-GOLD 120 C18 column (250 × 4.6 mm, 5 µm), 10-90-10% MeCN/H 2 O, flow rate: 1 mL/min] and [ 18 F] PPY2 [ReproSil-Pur 120 C18-AQ column (250 × 4.6 mm, 5 µm) 10–90–10% MeCN/H 2 O, flow rate: 1 mL/min].

Article Snippet: The purity of all the tested compounds was ≥95% as determined by an LC-MS system including a DAD detector [Dionex Ultimate 3000 system incorporating a LPG-3400SD pump, a WPS-3000 TSL autosampler, a TCC-3000SD column compartment, a DAD 3000 diode array detector and a MSQ Plus low resolution mass spectrometer (Thermo Fisher Scientific Inc., Waltham, MA, USA), column: Reprosil-Pur Basic HD (150 × 3 mm; 3 µm; Dr. Maisch GmbH, Ammerbuch, Germany), gradient: 10–90–10% MeCN/ 20 mM NH 4 OAc aq. ( v/v ), run time: 15 min, flow rate: 0.6 mL/min, UV-detection: 254 nm].

Techniques: In Vivo